amino-acid-hplc-buffers-solutions

HPLC Buffers & Solutions for Amino Acid Analysis

 

High-Quality HPLC Mobile Phases, Buffers & Solutions for Amino Acid Testing

High-Performance Liquid Chromatography (HPLC) is a widely used analytical technique for the separation, identification, and quantitative determination of amino acids in pharmaceutical products, food and beverages, biological samples, biotechnology applications, and research laboratories.

Reliable amino acid analysis by HPLC depends on the compatibility and quality of the complete analytical system, including the HPLC instrument, analytical column, detector, solvents, mobile phase, buffers, and, when required, derivatization reagents.

HPLC Solutions for Amino Acid Analysis

We provide a range of HPLC-grade solvents, buffers, mobile phases, and prepared solutions suitable for different amino acid analysis applications.

Our solutions can be used for the analysis of a wide range of amino acids, including:

  • Alanine
  • Arginine
  • Aspartic Acid
  • Glutamic Acid
  • Glycine
  • Glycine
  • Histidine
  • Isoleucine
  • Leucine
  • Lysine
  • Methionine
  • Phenylalanine
  • Proline
  • Serine
  • Threonine
  • Tryptophan
  • Tyrosine
  • Valine
  • Other amino acids and related compounds depending on the analytical method

HPLC Mobile Phases & Buffers for Amino Acid Analysis

The selection of the appropriate HPLC mobile phase and buffer is essential for achieving efficient amino acid separation, good peak shape, reproducible retention times, and accurate quantitative results.

Depending on the analytical method, amino acid HPLC analysis may use different combinations of HPLC-grade water, acetonitrile, methanol, phosphate buffers, acetate buffers, citrate buffers, organic acids, and other compatible reagents.

Some amino acid HPLC methods also require pre-column or post-column derivatization to improve detection and analytical sensitivity. The appropriate reagents and chromatographic conditions depend on the selected method, column chemistry, detector, and sample matrix.

Amino Acid Analysis Using HPLC

HPLC-based amino acid analysis is widely used in:

Pharmaceuticals | Food & Beverages | Nutritional Products | Clinical Research | Biotechnology | Protein & Peptide Analysis | Quality Control | Research Laboratories

With the appropriate HPLC system, analytical column, mobile phase, and detection method, amino acids can be separated and quantified with high reproducibility and analytical reliability.

Complete HPLC Solutions for Amino Acid Analysis

We can provide products and consumables for complete amino acid chromatography applications, including:

HPLC Systems | Amino Acid Analytical Columns | HPLC-Grade Solvents | Buffers & Mobile Phases | Derivatization Reagents | Prepared Solutions | HPLC Consumables | Deuterium Lamps

Our technical team can help you select suitable HPLC buffers, mobile phases, solvents, and amino acid analysis solutions according to your analytical method, HPLC configuration, column, and detector.

Contact us for information about HPLC solutions for amino acid analysis and other pharmaceutical, food, biotechnology, and laboratory applications.